Transcriptome analysis of the liver of Eospalax fontanierii under hypoxia

Hypoxia can induce cell damage, inflammation, carcinogenesis, and inhibit liver regeneration in non-adapted species. Because of their excellent hypoxia adaptation features, subterranean rodents have been widely studied to clarify the mechanism adaptation. Eospalax fontanierii , which is a rodent found China, survive for more than 10 h under 4% O 2 without observable injury, while Sprague-Dawley rats less 6 same conditions. To explore potential responses E. we performed RNA-seq analysis exposed different oxygen levels (6.5% 6h, 10.5% 44h, 21%). Based on bioinformatics analysis, 39,439 unigenes were assembled, 56.78% annotated using public databases (Nr, GO, Swiss-Prot, KEGG, Pfam). In total, 725 differentially expressed genes (DEGs) identified response hypoxia; six with important functions validated by qPCR. Those DEGs mainly involved processes related lipid metabolism, steroid catabolism, glycolysis/gluconeogenesis, AMPK PPAR signaling pathway. By analyzing expression patterns energy associated metabolism hypoxia, that fatty acid oxidation gluconeogenesis increased, protein synthesis decreased. Furthermore, upregulated specific anti-apoptosis or anti-oxidation may contribute tolerates hypoxia. Our results provide an understanding value biomedical studies.